Localization of the H-protein in H-proteinDd2 cells: Co-localization of GFP fluorescence from H-proteinDd2 cells with mitotracker and Hoechst dyes. Live cells from the H-proteinDd2 cell line were incubated with 100 nM mitotracker CMXRos (Invitrogen) and 1 μg/ml Hoechst stain for 20 minutes at 37°C followed by 2 washout

periods in complete media for five minutes. Cells were imaged on a Nikon Eclipse E800 epifluorescent microscope, and the images were processed with ImageJ software. The H-protein is localized exclusively to the mitochondrion .

Spalding MD, Allary M, Gallagher JR, Prigge ST. Validation of a modified method for Bxb1 mycobacteriophage integrase-mediated recombination in Plasmodium falciparum by localization of the H-protein of the glycine cleavage complex to the mitochondrion. Mol Biochem Parasitol. 2010 172(2):156-60. Copyright Elsevier