(D) IF using the PfNop1 and PfSir2 antibodies. The PfSir2 antibody is visualized in red and the PfNop1 antibody in green using Alexa 488. (E) Simultaneous IF and FISH analysis. Immunolocalization (IF) of PfSir2 was performed on young trophozoite parasites using an Alexa 488-conjugated secondary antibody (green). FISH analysis was performed simultaneously using a telomeric oligonucleotide probe conjugated with Cy3 (red). The nuclei were stained with DAPI. Protein localizes to telomeric foci. anti-PfSir2 antibody signal is highly concentrated at one pole of the nucleus.

Freitas-Junior LH, Hernandez-Rivas R, Ralph SA, Montiel-Condado D, Ruvalcaba-Salazar OK, Rojas-Meza AP, Mâncio-Silva L, Leal-Silvestre RJ, Gontijo AM, Shorte S, Scherf A. Telomeric heterochromatin propagation and histone acetylation control mutually exclusive expression of antigenic variation genes in malaria parasites. Cell. 2005 121:25-36. Copyright Elsevier 2009.