Confocal microscopy demonstrates patching of different ETRAMPs into distinct domains in formaldehyde-fixed IRBCs. IRBCs (D10E2myc parasites) coated on 10-well slides using concanavalinA (fixed with 1% formaldehyde and permeabilized with 0.03% saponin) were incubated with sera specific for ETRAMP2, ETRAMP10.1, ETRAMP2myc (detected via the tag) or anti-ETRAMP2 serum labelled with a fluorescent Fab fragment and viewed by confocal microscopy. A. ETRAMP2 (cy2, green) co-patches with ETRAMP2myc (cy3, red) into distinct areas in the PVM of several ring-stage IRBCs. B. ETRAMP10.1 (cy2, green) patches into distinct areas when compared with ETRAMP2myc (cy3, red) in several ring-stage IRBCs. bf, brightfield; bar size, 4 μm.

Spielmann T, Gardiner DL, Beck HP, Trenholme KR, Kemp DJ. Organization of ETRAMPs and EXP-1 at the parasite-host cell interface of malaria parasites. Mol Microbiol. 2006 59:779-94. PMID: