Regions of SURFIN4.1 required for the export to the Maurer's clefts. (A) Schematic drawings of the recombinant proteins, rSURFIN4.1 NCVT (NCVT), rSURFIN4.1 NCV (NCV), rSURFIN4.1 NCT (NCT), and rSURFIN4.1 N-T (N-T) are depicted in the left panels. The right panels show co-localization of a series of recombinant SURFIN4.1 detected with anti-Ty antibody (α-Ty) and counter-staining with PfSBP1 (α-SBP1) by IFA. The differential interference contrast (DIC) and merged images with nucleus stained with DAPI (Merge) are shown. (+) or (−) indicates positive or negative detection of the signals in the iRBC cytosol, respectively. Bar=5 μm. dotted fluorescence signal pattern in the iRBC cytosol, which co-localized with a MC marker PfSBP1. TM region is essential for export from the parasite to the PV lumen

Zhu X, Yahata K, Alexandre JS, Tsuboi T, Kaneko O. The N-terminal segment of Plasmodium falciparum SURFIN4.1 is required for its trafficking to the red blood cell cytosol through the endoplasmic reticulum. Parasitol Int. 2012 62(2):215-229.