Indirect immunofluorescence assay of three mini-SURFIN4.2 proteins. Double staining IFA for 3 mini-SURFIN4.2-expressing transfectants is shown. α-GFP and α-SBP1 indicate anti-GFP (mini-SURFIN4.2) and anti-SBP1 (Maurer's cleft protein). Negative controls using normal rabbit antibody did not produce detectable signals (not shown). CRD, cysteine-rich domain; TM, transmembrane; and Var1, variable region 1; WR, tryptophan-rich.

Alexandre JS, Yahata K, Kawai S, Torii M, Kaneko O. PEXEL-independent trafficking of Plasmodium falciparum SURFIN(4.2) to the parasite-infected red blood cell and Maurer's clefts. Parasitol Int. 2011 60(3):313-20.