Indirect immunofluorescence of P. falcparum-infected erythrocytes using anti-TCTP antibody. Fixed culture containing trophozoites were stained with anti-TCTP Ab + FITC-labled goat anti rabbit Ab and counterstained with DAPI. a) fluorescence; b) Light microscopy; c) DAPI; d) a late trophozoite showed FITC fluorescence only in discrete vacuole; e) which can be seen to contain hemozoin; f) but to be different from nuclei; g-h) purified food vacuoles also show FITC fluorescence. FV – food vacuole. N – nucleus. P – parasite. Bar – 10 mm. TCTP was found to be located in both the cytoplasm and the food vacuole.
Bhisutthibhan J, Philbert MA, Fujioka H, Aikawa M, Meshnick SR. The Plasmodium falciparum translationally controlled tumor protein: subcellular localization and calcium binding. Eur J Cell Biol. 1999 78:665-70. Copyright Elsevier 2011.