Immunogold EM localization of Pghl/PfMDR1. Asynchronous cultures of the cloned line ITG2F6 were analyzed by postembedding immunolabeling as described in Materials and Methods. The antibody used was the affinity-purified anti-Pghl followed by protein A conjugated to 10-nm gold particles. A trophozoite is shown on the left and a free digestive vacuole on the fight. Bars, 0.5 mm.
Cowman AF, Karcz S, Galatis D, Culvenor JG. A P-glycoprotein homologue of Plasmodium falciparum is localized on the digestive vacuole. J Cell Biol. 1991 113:1033-42. PMID: