IFA location of MSP119 during schizogony. Each row shows an identical field from 1% formaldehyde-fixed thin smears of late-stages parasites at the 1-, 2-, 4-, and 8-nuclei stage. Panel A1 shows anti-MSP119(rabbit) antibody labeling (the secondary antibody was Alexa Fluor 488-conjugated anti-rabbit IgG antibody (green). Panel A2 shows the same fields merged with the corresponding DAPI stained nucleus images (blue). Panel A3 shows the same fields visualized by light microscopy - note the pigment is clearly visible. Rabbit-anti-MSP119 antibody only detects MSP119 associated with the food vacuole until the two-nuclei stage. Once new full length MSP1 is synthesized and transported to the parasite’s plasma membrane the presence of MSP119 from the previous cycle is now obscured.

Dluzewski AR, Ling IT, Hopkins JM, Grainger M, Margos G, Mitchell GH, Holder AA, Bannister LH. Formation of the food vacuole in Plasmodium falciparum: a potential role for the 19 kDa fragment of merozoite surface protein 1 (MSP1(19)). PLoS ONE. 2008 Aug 3(8):e3085. PMID: