A and B. GFP fluorescence in live 3D7 (A) or D10 (B) parasites expressing wild-type REX2GFP from an episomal vector. GFP fluorescence appears as dots in the host cell cytoplasm typical for Maurer’s cleft localization. Nuclei are stained with DAPI (blue); merges with bright field images (bf) are shown. D. IFA of acetone-fixed infected RBCs expressing REX2GFP and stained with anti-GFP (green) and anti-KAHRP (red) antibodies. Overlays are shown as indicated above each panel. The last panel shows an overlay of all three images (merged). E. IFA of acetone-fixed infected RBCs expressing REX2GFP and stained with anti-GFP (green) and anti-BiP (red) antibodies. Overlays of panels are shown as in (D). Size bars are 2 mm.

Haase S, Herrmann S, Grüring C, Heiber A, Jansen PW, Langer C, Treeck M, Cabrera A, Bruns C, Struck NS, Kono M, Engelberg K, Ruch U, Stunnenberg HG, Gilberger TW, Spielmann T. Sequence requirements for the export of the Plasmodium falciparum Maurer's clefts protein REX2. Mol Microbiol. 2009 71:1003-17. Copyright John Wiley & Sons Ltd. 2010.