Immunofluorescence assays of P. falciparum gametocytes at 15 min post-activation showed PPLP2 labelling (green) in association with shed membranes (indicated by arrows). The exflagellating microgametocyte is highlighted by labelling of Pfs25 (red). Arrowheads indicate microgametes. The corresponding differential interference contrast (DIC) images are shown. Bar, 5 μm.

Deligianni E, Morgan RN, Bertuccini L, Wirth CC, Silmon de Monerri NC, Spanos L, Blackman MJ, Louis C, Pradel G, Siden-Kiamos I. A perforin-like protein mediates disruption of the erythrocyte membrane during egress of Plasmodium berghei male gametocytes. Cell Microbiol. 2013 15(8):1438-55