PF3D7_0600400 erythrocyte membrane protein 1, PfEMP1

Immunofluorescence assays with variant-specific antibodies. An immunofluorescence assay (IFA) was performed using anti-PF3D7_0100300-His, anti-PF3D7_0600400-His, and anti-PF3D7_0937600-His IgG (1:50) as primary antibodies and anti-ATS IgG as the control. Specific staining of infected erythrocytes (IEs) is observed as punctuated fluorescence patterns over the IE surface using a secondary antibody labeled with Alexa 488 (green). The pre-immune sera of the immunized rats did not result in any surface reactivity with the IE. DAPI (5 mg/mL) staining of DNA in the nuclei is blue. The anti-ATS IgG and anti-PF3D7_0600400-His IgG stained IEs of both the trophozoite and schizont stages, the anti-PF3D7_0937600-His IgG only reacted with IEs of the trophozoite stage, while the anti-PF3D7_0100300-His IgG only reacted with IEs of the schizont stage.

Zhang Y, Jiang N, Lu H, Hou N, Piao X, Cai P, Yin J, Wahlgren M, Chen Q. Proteomic analysis of Plasmodium falciparum schizonts reveals heparin-binding merozoite proteins. J Proteome Res. 2013 May 3;12(5):2185-93.

Other associated proteins

PFID Formal Annotation
PF3D7_0937600 erythrocyte membrane protein 1, PfEMP1
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