Immunolocalization of PfSEA-1. (C) Nonpermeabilized, unfixed schizont-infected RBCs were probed with mouse anti–PfSEA-1 (red) and rabbit anti–glycophorin A (green) and counterstained with DAPI to label parasite nuclei. PfSEA-1 colocalized with glycophorin A to the surface of schizont-infected RBCs. Scale bar, 5 mm. (D to F) Nonpermeabilized, unfixed schizontinfected RBCs were probed with mouse anti–PfSEA-1 (5-nm gold particles) and rabbit anti–glycophorin A (10-nm gold particles). PfSEA-1 localized to the schizont/parasitophorous vacuole membrane (black arrow), Maurer's clefts (yellow arrow), and the inner leaflet of the RBC membrane (gray arrow), whereas glycophorin A was confined to the outer leaflet of the RBC membrane (white arrow). Scale bar in (D), 0.5 mm. Scale bars in (E) and (F), 0.1 mm.

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