PfPTP1 is localised on Maurer’s clefts in P. falciparum-infected RBCs. (A) Immunofluorescence assay on CS2/PfPTP1-GFP cell line: the GFP signal (green) colocalises with that of the endogenous protein (PfPTP1; red); inset: enlargement of colocalisation pattern. (B) Immuno-fluorescence assay on CS2/PfPTP1-HA cell line: the HA signal (green) colocalises with the α-PfPTP1 signal (red); inset: enlargement of colocalisation pattern. (C) Immunofluorescence assay on CS2/PfPTP1-HA cell line: the HA-signal (green) colocalises partially with the MC resident protein SBP1 (red); inset: enlargement of colocalisation pattern. (D) Immunofluorescence assay on CS2 parental cell line: the PfPTP2-signal (green) does not overlap with the PfPTP1-signal (red); inset: enlargement of area where MC (PfPTP1) and electron dense vesicles (PfPTP2) are in close proximity. (E) Immunofluorescence assay on CS2 parental cell line: the PfHsp70-x-signal (green) colocalises partially with the PfPTP1- signal (red); inset: enlargement of partial colocalisation on J-Dots (PfHsp70-x). Scale bar: 1μm; same size of ROI in each image.

Rug M, Cyrklaff M, Mikkonen A, Lemgruber L, Kuelzer S, Sanchez CP, Thompson J, Hanssen E, O'Neill M, Langer C, Lanzer M, Frischknecht F, Maier AG, Cowman AF. Export of virulence proteins by malaria-infected erythrocytes involves remodelling of host actin cytoskeleton. Blood. 2014 Aug 19 [Epub ahead of print]