Over-expression of sensitive and resistant form of PfCRT as GFP-fusion protein. Fluorescence microscopy of GFP-fusions of sensitive (PfCRTS, top) and resistant (PfCRTR, bottom) form of PfCRT. PfCRTS and PfCRTR were over-expressed as GFP-fusion proteins using an ATc-inducible expression system. Live cell images of DAPI-stained infected red blood cells show that both forms of PfCRT localise to the FV membrane. Later stages of the asexual life cycle show ring/dot-like structures (white arrow) within the FV, possibly degraded GFP-fusion proteins.
Ehlgen F, Pham JS, de Koning-Ward T, Cowman AF, Ralph SA. Investigation of the Plasmodium falciparum food vacuole through inducible expression of the chloroquine resistance transporter (PfCRT). PLoS One. 2012;7(6):e38781.