Localization of native PfRh4 was assessed by wide-field immunofluorescence assay using the anti-PfRh4 mAbs. PfRh4 (green) was co-stained with rhoptry protein, PfRh2 (red) and nuclei-stain DAPI (blue). DIC shows the differential interference contrast view of the same field. Scale bar = 5 μm. PfRh4, together with the other PfRh family of proteins, localize to the rhoptries of P. falciparum parasites in an apical localization that predominantly co-localized with PfRh2 another rhoptry protein.

Lim NT, Harder MJ, Kennedy AT, Lin CS, Weir C, Cowman AF, Call MJ, Schmidt CQ, Tham WH. Characterization of Inhibitors and Monoclonal Antibodies that Modulate the Interaction between Plasmodium falciparum Adhesin PfRh4 with its Erythrocyte Receptor Complement Receptor 1. J Biol Chem. 2015 Aug 31. [Epub ahead of print]