Transmission electron microscopy analysis of REX1-GFP and REX1(Δ371–579)-GFP Maurer’s clefts. A,B. TEM images of glutaraldehyde-fixed REX1-GFP and REX1(Δ371–579)-GFPinfected RBCs (100 nm sections) revealing unstacked and highly stacked Maurer’s clefts (MC). lamellae. C,D. TEM images of EqtII-permeabilized samples showing single Maurer’s cleft lamellae and stacked lamellae (100 nm sections). Knobs (K) are indicated. The REX1-GFP parasites displayed an unstacked Maurer's cleft phenotype (A,C) equivalent to that previously reported for the 3D7 strain Dramatically, the REX1Δ371-579-GFP parasites showed very large stacks of Maurer's clefts (B).

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