A CaaX motif is required for FCP localization. Live epifluorescent images of P. falciparum parasites expressing either Wt GFP-FCP (top panel) or GFP-FCP(C322S) (bottom panel). The nucleus was stained using Hoechst. GFP-FCP showed fluorescence overlapping or surrounding parasite hemozoin, consistent with its previously reported food vacuole localization. However, GFP-FCP(C322S) fluorescence appeared throughout the cell consistent with cytosolic mislocalization.

Gisselberg JE, Zhang L, Elias JE, Yeh E. The prenylated proteome of Plasmodium falciparum reveals pathogen-specific prenylation activity and drug mechanism-of-action. Mol Cell Proteomics. 2016 Dec 31. pii: mcp.M116.064550. PMID: 28040698.