An artificial N-terminal extension has no effect on protein export efficiency. (B) Addition of the GBP130 N-terminal extension or an artificial extension to a canonical signal peptide has little effect on protein export efficiency. A schematic of each construct is shown above the respective images. All images are of live cells. DIC, differential interference contrast; GFP, green fluorescent protein; Hoechst, nuclear staining. In merge and overlay: green, GFP; blue, Hoechst.

Meyer C, Barniol L, Hiss JA, Przyborski JM. The N-terminal extension of the P. falciparum GBP130 signal peptide is irrelevant for signal sequence function. Int J Med Microbiol. 2017 S1438-4221(17)30195-9.