PfHsp70x knockdown does not inhibit export of virulence-associated proteins. Asynchronous PfHsp70x-M9 and PfHsp70x-glmS parasites were fixed with acetone (PfFIKK4.2 and MAHRP1) or paraformaldehyde

(KAHRP) and stained with antibodies against PfFIKK4.2 (A), KAHRP (B), or MAHRP1 (C). DAPI was used to mark parasite cell nucleus. From left to right, the images are phase-contrast micrographs of parasites, parasites stained with DAPI (blue), parasites stained with antibody against the exported protein (green), parasites stained with anti-HA antibody (red), and fluorescence and phase-contrast merge images of the parasites. Representative images are shown. Bars, 5 mm. in both PfHsp70-KO and PfHsp70x-glmS mutants, export of parasite virulence factors is not affected,

Cobb DW, Florentin A, Fierro MA, Krakowiak M, Moore JM, Muralidharan V. The Exported Chaperone PfHsp70x Is Dispensable for the Plasmodium falciparum Intraerythrocytic Life Cycle. mSphere. 2017 2(5). pii: e00363-17