(C) and (D) Immuno-colocalization of PfCAF1 in different stages of IDC using pre-immune and immune antibodies, respectively. DNA was stained with DAPI (blue). The bar indicates 1 mm. Pre-immune sera failed to detect any signal under similar experimental conditions. The increase of punctate foci from ring to schizont stage supports the results obtained from stage specific western blot analysis. The punctate pattern of PfCAF1 signal around nucleus suggests its nuclear localization.

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