RASP2 plays an essential role in rhoptry secretion. IFA on E64 or DMSO-treated iKO-PfRASP2-HA3 parasites ± rapamycin. PfRASP2 depletion does not affect PfAMA1 relocalisation at the surface of merozoites (green arrows). PfAMA1 translocation from the micronemes to the plasma membrane of fully mature daughter merozoites during egress suggesting that the rhoptry secretion defect observed is independent of the ability of the mutant to secrete proteins from micronemes.

Suarez C, Lentini G, Ramaswamy R, Maynadier M, Aquilini E, Berry-Sterkers L, Cipriano M, Chen AL, Bradley P, Striepen B, Boulanger MJ, Lebrun M. A lipid-binding protein mediates rhoptry discharge and invasion in Plasmodium falciparum and Toxoplasma gondii parasites. Nat Commun. 2019 10(1):4041.