GEXP07-GFP is not detectable at the infected RBC membrane. (A) Whole-cell immunofluorescence of GEXP07-GFP parasites labeled with anti-KAHRP (green) and anti-GFP (green). Nuclei were stained with DAPI (blue). Projections of Z stacks are shown. Scale bar=5 mm. (B) Sheared remnant membranes from RBCs infected with 3D7 and GEXP07 were labeled with anti-KAHRP (red) and anti-GFP (green). CellMask (CM) reveals each of the membrane discs in the sample (gray). Scale bar=5 mm. The fluorescence profile for GEXP07-GFP is quite different from that of the RBC membrane-associated protein, KAHRP.

McHugh E, Carmo OMS, Blanch A, Looker O, Liu B, Tiash S, Andrew D, Batinovic S, Low AJY, Cho HJ, McMillan P, Tilley L, Dixon MWA. Role of Plasmodium falciparum Protein GEXP07 in Maurer's Cleft Morphology, Knob Architecture, and P. falciparum EMP1 Trafficking. mBio. 2020 11(2). pii: e03320-19.