DiQ-BioID of PfCERLI1 and PfCERLI2 reveals their protein interactome. b Pfcerli1DiQ-BioID and Pfcerli2DiQBioID parasites were treated for 8 h on rapalog to permit for dimerisation between the GFP-tagged bait construct and mCherry-tagged BirA, visualised by live fluorescence. Scale bars =2 μm. c Biotinylation at the rhoptry bulb was confirmed by co-localising foci between RAP1 staining and a streptavidin-conjugated AlexaFluor488 in fixed cells by IFA. Scale bars= 2 μm.

Liffner B, Balbin JM, Shami GJ, Siddiqui G, Strauss J, Frölich S, Heinemann GK, Edwards EM, Alder A, Wichers JS, Creek DJ, Tilley L, Dixon MWA, Gilberger TW, Wilson DW. Cell biological analysis reveals an essential role for Pfcerli2 in erythrocyte invasion by malaria parasites. Commun Biol. 2022 9;5(1):121.