Electron micrographs of gametocytes (G) and the infected erythrocytes (E) at 5 min after induction of gametogenesis, embedded in Epog (Fig. 3 and 4) or LR White (Fig. 5).F,16. 3. Clear spaces are prominent in the erythrocyte cytoplasm. No membranes surround these spaces. A pellicular complex consists of gametocyte plasmalemma (GPM) and an inner layer composed of two closely apposed membranes (IL). Note the absence of parasitophorous vacuolar membrane. Bar, 0.1 ,um. FIG. 4. In some regions surrounding the pellicular complex are multilamellar membranes (arrows) presumably derived from the vacuolar membrane. Clear spaces without membrane are seen throughout the erythrocyte hemoglobin-rich cytoplasm. Bar, 0.1 ,um.FIG. 5. Immuno-gold labeling with human MAb 33G2. Gold particles are associated with hemoglobin surrounding the clear spaces (S).Bar, 0.5 um. Quakyi IA, Matsumoto Y, Carter R, Udomsangpetch R, Sjolander A, Berzins K, Perlmann P, Aikawa M, Miller LH. Movement of a falciparum malaria protein through the erythrocyte cytoplasm to the erythrocyte membrane is associated with lysis of the erythrocyte and release of gametes. Infect Immun. 1989 PMID: 2645212;