Mitochondria are taken up into early endosomes in response to the mitochondrial recruitment of ATG16L1[100-250]. (A) ARPE-19 cells stably expressingHA-RAB5AQ79L and mitochondrially localized mCherry-FIS1[101-152] were transduced to express the indicated proteins, or were treated with DFP (1 mM), for 48 h before immunofluorescence staining with anti-HA and confocal microscopy analysis. Representative images are shown. Scale bar: 5 µm. Artificial targeting of autophagy components to mitochondria reveals both conventional and unconventional mitophagy pathways, Lorentzen KC, Prescott AR, Ganley IG. Artificial targeting of autophagy components to mitochondria reveals both conventional and unconventional mitophagy pathways. Autophagy. 2025.