RAP2 is located in the ER lumen of ΔRAP1 parasites. Individual rows show mature schizonts of D10 and ΔRAP1 parasites (D10ΔRAP1c1) as labelled. Each row shows staining with polyclonal rabbit anti-PfERC (red) and anti-RAP1 (green) or staining with monoclonal anti-RAP2 (green). Merged images are PfERC + RAP1 or RAP2 (yellow indicates co-localization) for the same parasite. Top panel: PfERC and RAP1 do not co-localize in D10 schizonts. Second panel: PfERC and RAP1 do not co-localize in ΔRAP1 schizonts. Third panel: PfERC and RAP2 do not co-localize in D10 schizonts. Fourth panel: PfERC and RAP2 co-localize in ΔRAP1 schizonts. Baldi DL, Andrews KT, Waller RF, Roos DS, Howard RF, Crabb BS, Cowman AF. RAP1 controls rhoptry targeting of RAP2 in the malaria parasite Plasmodium falciparum. EMBO J. 2000 19(11):2435-43. PMID: 10835342;