Surface GPI-anchored proteins lose their membrane association in second cycle partially rescued delayed death (PRDD) P. falciparum. A) The localisation of surface GPI-anchored proteins was examined along with proteins associated with membranes via dolichol independent mechanisms. The GPI-anchored protein merozoite surface protein 2 (MSP2) (green) was co-localised (red) with B) GPI-anchored MSP1 C) transmembrane protein apical membrane antigen (AMA1), D) membrane spanning pore exported protein 2 (EXP2) and E) glideosome associated protein 45 (GAP45)–membrane associated by attachment of palmityl and myristoyl groups. Apicoplast inhibition with translation inhibitors results in surface GPI-anchored proteins lose their membrane association. Nuclei stained with 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar = 5 μm. Bulloch MS, Huynh LK, Kennedy K, Ralton JE, McConville MJ, Ralph SA. Apicoplast-derived isoprenoids are essential for biosynthesis of GPI protein anchors, and consequently for egress and invasion in Plasmodium falciparum. PLoS Pathog. 2024 20(9):e1012484. PMID: 39241090.