Growth and fission of the apicoplast.

Parasites expressing GFP-conjugated to the apicoplast transit signal of ACP (ACPTs-GFP) were prepared by ultrastructural expansion microscopy (U-ExM), stained with N-hydroxysuccinimide (NHS) ester (grayscale), BODIPY TRc (white), SYTOX (cyan), and anti-GFP (apicoplast) (magenta) antibodies and using Airyscan microscopy. (a) Images of whole parasites throughout asexual blood-stage development. Maximum-intensity projections of both a subsection of the cell (partial mito) and the full cell (full mito) are shown. (c) Representative images of the different stages of apicoplast fission. Images are maximum-intensity projections, number on image = Z-axis thickness of projection in µm. Asterisks represent centriolar plaques. Scale bars = 2 µm.

Anaguano D, Frölich S, Muralidharan V, Wilson DW, Dvorin J, Absalon S. Liffner B, Cepeda Diaz AK, Blauwkamp J, Anaguano D, Frolich S, Muralidharan V, Wilson DW, Dvorin JD, Absalon S. Atlas of Plasmodium falciparum intraerythrocytic development using expansion microscopy. Elife. 2023 24:2023.03.22.533773.