Immunofluorescence images show PfTPx Gl , microtubules, ACP-GFP, PfACP and PfEMP1 trafficking in AlF 4-treated D10-ACPleader-GFP parasites.

(A) PfTPx Gl, ACP-GFP, PfACP and PfEMP1 localization in control parasites, (B) PfTPx Gl, ACP-GFP, PfACP and PfEMP1 localization in AlF4-treated parasites. For D10-ACPleader-GFP parasites, 98% of the 115 parasites analyzed showed disrupted PfTPx Gl signal while 96% of the 147 parasites analyzed showed arrest of PfEMP1 in the parasites. Scale Bar: 10 µm In ACP-GFP panel, upper band indicated with an arrowhead represents an unprocessed form of ACP-GFP while lower band represents processed form of ACP-GFP. Chaudhari R, Dey V, Narayan A, Sharma S, Patankar S. Membrane and luminal proteins reach the apicoplast by different trafficking pathways in the malaria parasite Plasmodium falciparum. PeerJ. 2017 Apr 27;5:e3128. doi: 10.7717/peerj.3128. PMID: 28462015;