A full-length C-terminus is required for antigen delivery and wildtype cleft morphology. conjugated to Alexa Fluor 647. Samples were run in technical duplicates. Data displayed are mean fluorescence values±SD for each biological repeat. 4 biological repeats are displayed, each with their own shape. (B) Trypsin cleavage assay of truncation lines. Membranes were probed with αATS and the loading/experimental controlαSBP1. P: PBS mock treatment; T: Trypsin treated samples; asterisk: spectrin cross-reactivity band; arrows: Trypsin cleaved EMP1 products. Loading control and experimental control,αSBP1 expected molecular weight is ~50 kDa. ((A) Flow cytometry analysis of infected RBCs labeled with antibodies to the ectodomain of var2CSA followed by secondary antibodies and tertiary antibodies C) Equinatoxin-II permeabilized infected RBCs probed with αGFP followed by immunogold secondary labeling. (D-E) Quantitation of vesicles from immuno-electron micrographs. (D) Mean vesicle diameter per image. Data displayed are mean±SD (n= 32, 41, 24, 24 respectively). (E) Number of vesicles within 100 nm of each cleft. Data displayed are mean±SD (n= 43, 73, 38, 42 respectively). P-values determined by Brown-Forsythe and Welch ANOVA tests and Dunnett’s multiple comparison test,nvalues are individual cells. Carmo OMS, Shami GJ, Cox D, Liu B, Blanch AJ, Tiash S, Tilley L, Dixon MWA. Deletion of the Plasmodium falciparum exported protein PTP7 leads to Maurer's clefts vesiculation, host cell remodeling defects, and loss of surface presentation of EMP1. PLoS Pathog. 2022 Aug 5;18(8):e1009882. doi: 10.1371/journal.ppat.1009882. PMID: 35930605