Subcellular localization of PfUSP in transgenic parasites. (A) Fluorescent images of PfUSP-HA-glmS transgenic parasites at trophozoite and schizont stages (grown in absence of glucosamine) immuno-stained with anti-HA antibody (green) and anti-BiP antibody (red). The anti-HA labelling showed overlap with ER labelling by anti-BiP antibody in distinct locations. (B) Fluorescent images of PfUSP-HA-glmS transgenic parasites at trophozoite and schizont stages (grown in absence of glucosamine) immuno-stained with anti-HA antibody (green) and labelled with MitoTracker (red); immunostaining of anti-HA 2 antibody was distinct from MitoTracker labelling. (C) Fluorescent images of PfUSP-HA3 glmS transgenic parasites at trophozoite and schizont stages (grown in absence of glucosamine) immuno-stained with anti-HA antibody (green) and anti-PfClpP (labelling apicoplast targeted protein PfClpP) (red); immunostaining of anti-HA antibody was distinct from apicoplast labelling. The parasite nuclei were stained with DAPI (blue) and parasites were visualized by confocal laser scanning microscope.
Caradec T, Trivelli X, Desmecht E, Peucelle V, Khalife J, Hartkoorn RC. Dactylosporolides: Glycosylated Macrolides from Dactylosporangium fulvum. J Nat Prod. 2022
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_0403500 | ubiquitin specific protease, putative |
PF3D7_0917900 | PfHsp70-2 |