pfcarl is localized to cis-Golgi apparatus of P. falciparum asexual- and sexual-stage parasites. Immunofluorescence colocalization of PfCARL and ERD2 in two representative P. falciparum asexual parasites. BF, brightfield; DAPI, 4’, 6-diamidino-2-phenylindole; Trophs, trophozoites; PfCARL, antibody against PfCARL; aERD2, antibody against ER domain protein #2. The PfCARL overexpressing strains demonstrated colocalization of PfCARL with an anti-ERD2 (ER domain protein no. 2) antibody in several different asexual blood stages, which strongly indicated that PfCARL is localized to the cis-Golgi apparatus of the parasite.

LaMonte G, Lim MY, Wree M, Reimer C, Nachon M, Corey V, Gedeck P, Plouffe D, Du A, Figueroa N, Yeung B, Bifani P, Winzeler EA. Mutations in the Plasmodium falciparum Cyclic Amine Resistance Locus (PfCARL) Confer Multidrug Resistance. MBio. 2016 Jul 5;7(4). pii: e00696-16.