Membrane localization of PfPLP1 was detected using IFA of untreated and BAPTA-AM-treated schizonts. Schizonts were stained with anti-PfPLP1 (green) and membrane dye (red) and visualized under confocal microscope. Three-dimensional reconstruction from z-stacks of IFA images using Imaris software is shown. PfPLP1 is localized on the RBCM of untreated schizonts but not in BAPTA-AM (B/AM)-treated schizonts, indicating that intracellular Ca2+ chelation blocks membrane localization of PfPLP1. Garg S, Agarwal S, Kumar S, Yazdani SS, Chitnis CE, Singh S. Calcium-dependent permeabilization of erythrocytes by a perforin-like protein during egress of malaria parasites. Nat Commun. 2013. PMID: 23591903.