N-terminal lipid modification motifs are essential for PfARO rhoptry membrane association. Schematic representation Pf ARO-GFP fusion protein and localization in unfixed parasites (schizonts and merozoites) of (A) wild type, (B) PfARO D20 mutant, (C) PfAROA2A5A6 (double acylation) mutant, (D) PfAROA2 (myristoylation) mutant and (E) PfAROA5A6 (palmitoylation) mutant. Nuclei stained with DAPI (blue). Enlargement of selected areas are marked with white square and referred as Zoom. Scale bar, 1 μm. Dissection of Minimal Sequence Requirements for Rhoptry Membrane Targeting in the Malaria Parasite. Cabrera A, Herrmann S, Warszta D, Santos JM, John Peter AT, Kono M, Debrouver S, Jacobs T, Spielmann T, Ungermann C, Soldati-Favre D, Gilberger TW. Dissection of minimal sequence requirements for rhoptry membrane targeting in the malaria parasite. Traffic. 2012 13(10):1335-50. PMID: 22759070.