Characterisation of intranuclear and subpellicular microtubules.

(a) 3D7 parasites were prepared by U-ExM, stained with NHS ester (greyscale), BODIPY TRc (white), SYTOX (cyan) and anti-tubulin (microtubules; magenta) antibodies, and imaged using Airyscan microscopy across the asexual blood stage. (b) Nuclei in the process of dividing, with their MTOCs connected by an interpolar spindle. (c) The number and type of microtubule branches in

interpolar spindles and (d) length of interpolar microtubules. (e) Subpellicular microtubules (SPMTs) stained with an anti-poly glutamylation (PolyE; yellow) antibody. (f) Quantification of the number of SPMTs per merozoite from C1-treated schizonts. (g) SPMT biogenesis throughout segmentation. (h) Model for SPMT biogenesis. PPM = parasite plasma membrane, APRs = apical polar rings, BC = basal complex. Images are maximum-intensity projections, number on image = Z-axis thickness of projection in µm. Scale bars = 2 µm.

Anaguano D, Frölich S, Muralidharan V, Wilson DW, Dvorin J, Absalon S. Liffner B, Cepeda Diaz AK, Blauwkamp J, Anaguano D, Frolich S, Muralidharan V, Wilson DW, Dvorin JD, Absalon S. Atlas of Plasmodium falciparum intraerythrocytic development using expansion microscopy. Elife. 2023 Dec 18;12:RP88088. doi: 10.7554/eLife.88088. PMID: 38108809;y. bioRxiv [Preprint]. 2023 24:2023.03.22.533773. PMID: 36993606;