PfRh5 is expressed in schizonts and merozoites and is refractory to knock-out but not C-terminal tagging. (A) Indirect immunofluorescence and phase contrast micrographs of late segmented schizonts and free merozoites using rabbit polyclonal serum raised against recombinant PfRh5. The panels from left to right are rabbit anti-Rh5, DAPI nuclear stain, phase contrast image and an overlay of all three images. Scale bars = 1 lM. (B) The sScheme for plasmid integration by single homologous cross-over recombination to add a single Strep-tag II and triple Haemaglutinin (HA) tag to the PfRH5 C-terminus. (C) Immunoblot with anti-Rh5 monoclonal (clone 6H2) against culture supernatant from wild type 3D7 and D10, and HA tagged parasite lines in both to detect PfRh5. The reactivity at ~70 kDa is cross-reactivity of antibody with serum albumin which is present at high concentration in the supernatant medium. (D) Immunoprecipitation of PfRh5 with rabbit polyclonal serum against PfRh5 (or pre-immune normal rabbit serum control) probed with PfRh5 monoclonal. Reticulocyte-binding protein homologue 5 – An essential adhesin involved in invasion of human erythrocytes by Plasmodium falciparum. Baum J, Chen L, Healer J, Lopaticki S, Boyle M, Triglia T, Ehlgen F, Ralph SA, Beeson JG, Cowman AF. Reticulocyte-binding protein homologue 5 - an essential adhesin involved in invasion of human erythrocytes by Plasmodium falciparum. Int J Parasitol. 2009.