PfSUMO immunofluorescence assays throughout the blood stage cycle. A. Reactivity of anti-PfSUMO sera on wild-type 3D7 strain parasites. B. Reactivity of anti-FLAG (tag) antibody on PfSUMO-FLAG-tagged parasites. Ring stage 10–14 h, trophozoite stage 26–30 h, schizont stage 40–46 h post invasion. C. Comparison of IFA pattern obtained by anti-PfSUMO and anti-FLAG antibodies on PfSUMO-FLAG-tagged parasites (trophozoites). Size bar corresponds to 1 μm. Nuclear staining by DAPI in blue.Issar N, Roux E, Mattei D, Scherf A. Identification of a novel post-translational modification in Plasmodium falciparum: protein sumoylation in different cellular compartments. Cell Microbiol. 2008 PMID: 1854733

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