Expression and localization of PfGAP40 in Plasmodium falciparum. Costaining of PfGAP40-Ty with PfGAP45 in ring (R), trophozoite (T), early schizont (ES), late schizont (LZ), free merozoite (MZ), and gametocyte (GC) of P. falciparum. PfGAP40-Ty colocalized with PfGAP45 and Pfs16 around the periphery of segmented schizonts, free merozoites, and gametocytes. In the merged color image, the PfGAP40-Ty signal is green, the antibody specific for the PfGAP45 or Pfs16 protein is red, and the parasite nuclei are stained with 4′,6-diamino-2-phenylindole (DAPI) in blue. The differential inference contrast (DIC) images are also shown. The Pearson’s colocalization coefficient value of the Ty/GAP45 or Ty/Pfs16 in PfGAP40cKD strain is shown in the right merged panel. Scale bars: 5 μm.

He L, Qiu Y, Pang G, Li S, Wang J, Feng Y, Chen L, Zhu L, Liu Y, Cui L, Cao Y, Zhu X. Plasmodium falciparum GAP40 Plays an Essential Role in Merozoite Invasion and Gametocytogenesis. Microbiol Spectr. 2023 11(3):e0143423.