IFA analysis of matured schizonts and merozoites from three GFP::PfGAP40 mutants (GFP::WT, GFP::3S-to-D, and GFP::3S-to-A). Specific GFP-2×FKBP-PfGAP40 fusion proteins were probed with anti-PfGAP45 (green) and anti-GFP (red) antibodies. BF, bright field. Nuclei are stained with DAPI (blue). Scale bar: 5 μm. Immunofluorescence assays and live fluorescent imaging of mature schizonts and released merozoites revealed that all GFP-2×FKBP-tagged PfGAP40 mutants were expressed and targeted correctly to the P. falciparum IMC.

He L, Qiu Y, Pang G, Li S, Wang J, Feng Y, Chen L, Zhu L, Liu Y, Cui L, Cao Y, Zhu X. Plasmodium falciparum GAP40 Plays an Essential Role in Merozoite Invasion and Gametocytogenesis. Microbiol Spectr. 2023 11(3):e0143423.