Costaining of PfGAP40-Ty with PfGAP45 in ring (R), trophozoite (T), early schizont (ES), late schizont (LZ), free merozoite (MZ), and gametocyte (GC) of P. falciparum. PfGAP40-Ty colocalized with PfGAP45 and Pfs16 around the periphery of segmented schizonts, free merozoites, and gametocytes. In the merged color image, the PfGAP40-Ty signal is green, the antibody specific for the PfGAP45 or Pfs16 protein is red, and the parasite nuclei are stained with 4′,6-diamino-2-phenylindole (DAPI) in blue. The differential inference contrast (DIC) images are also shown. He L, Qiu Y, Pang G, Li S, Wang J, Feng Y, Chen L, Zhu L, Liu Y, Cui L, Cao Y, Zhu X. Plasmodium falciparum GAP40 Plays an Essential Role in Merozoite Invasion and Gametocytogenesis. Microbiol Spectr. 2023 11(3):e0143423.