PfZIP1 detection and localization through intraerythrocytic stages. Immunofluorescence confocal microscopy of parasites at different stages using anti-PfZIP1 and anti-PfHU Abs. PfHU served as an apicoplast marker and 4′,6-diamidino-2-phenylindole (DAPI) as a nuclear dye. DIC: differential interference contrast. Images shown are representative of at least 30−50 images scanned for each stage from three sets of IFA labeling experiments.

Shrivastava D, Jha A, Kabrambam R, Vishwakarma J, Mitra K, Ramachandran R, Habib S. Plasmodium falciparum ZIP1 Is a Zinc-Selective Transporter with Stage-Dependent Targeting to the Apicoplast and Plasma Membrane in Erythrocytic Parasites. ACS Infect Dis. 2024 10(1):155-169.