Requirements for SEMP1 export into the RBC. A: Lysates of SEMP1-KO parasites expressing full-length and truncated or mutated forms ofSEMP1C-terminally fused to GFP were generated by saponin lysis and analysed by immunoblotting using mouse a-GFP antibodies. Lane1: SEMP11–123-GFP (full-length), lane2: SEMP117–123-GFP, lane3: SEMP172–123GFP, lane4: SEMP11–97-GFP, lane5: MSP11–16 SEMP1 17–123-GFP, lane6: Dietz O, Rusch S, Brand F, Mundwiler-Pachlatko E, Gaida A, Voss T, Beck HP. Characterization of the small exported Plasmodium falciparum membrane protein SEMP1. PLoS One. 2014 9(7):e103272.. PMID: 25062022