Knockout of SEMP1 by gene disruption has no detectable phenotype. C: Immunofluorescence assays of fixed RBCs infected with SEMP1-KO parasites, probed with a-MAHRP1, a-SBP1, a-REX1 and a-PfEMP1 antibodies. The parasite nuclei were stained with DAPI. D: Bar graph depicting differential binding to CD36 of RBCs infected with wild-type 3D7 and SEMP1-KO parasites. CD36 (or BSA as control) was immobilized on Petri dishes and bound infected RBCs (iRBCs) were calculated as iRBCs per mm2 for 1% parasitemia. . Dietz O, Rusch S, Brand F, Mundwiler-Pachlatko E, Gaida A, Voss T, Beck HP. Characterization of the small exported Plasmodium falciparum membrane protein SEMP1. PLoS One. 2014 9(7):e103272