Dynamics of Orc2 expression and its localization during the intraerythrocytic developmental (IED) stages. (C) IFA using antibodies against Orc2N shows a diffused extra-nuclear pattern. (D) IFA showing the punctate localization of PfOrc2C. (DAPI was used to stain nucleus; early parasitic stages have been shown for representation in C, D; Scale bar represents 2µm in each case). The top panels are shown in B/W and the bottom panels are shown in color. Indirect Immunofluorescence assay (IFA) using antibodies raised against PfOrc2N showed a diffused pattern around the nuclear DAPI stain in all parasite stages (Fig. 2C, S7A). IFA using PfOrc2C antibodies showed punctate perinuclear foci of PfOrc2 around the nucleus in early stage

Sharma R, Sharma B, Gupta A, Dhar SK. Identification of a novel trafficking pathway exporting a replication protein to nucleus via classical secretory pathway in Plasmodium falciparum. Biochim Biophys Acta. 2018 Mar 7. [Epub ahead of print]