Membrane dynamics of the Plasmodium falciparum digestive vacuole (DV). Shown are transgenic P. falciparum

parasites expressing endogenous chloroquine resistance transporter (CRT) fused to mNeonGreen. Depicted is the mNeonGreen signal (green, top) and a merge with differential interference contrast (DIC, bottom). (A) A parasite showing no DV lobes. (B) A parasite showing one lobe budding from the DV membrane (DVM). (C) A parasite harboring an autonomous CRTpositive compartment which is distinct from the DV. Detachment of DV lobes from the mother DV. Matz JM. Plasmodium's bottomless pit: properties and functions of the malaria parasite's digestive vacuole. Trends Parasitol. 2022 38(7):525-543. PMID: 35317985.