ZIP1 detection and localization through intraerythrocytic stages. (PfZIP1 detection and localization through intraerythrocytic stages. Immunofluorescence confocal microscopy of parasites at different stages using anti-PfZIP1 and anti-PfHU Abs. PfHU served as an apicoplast marker and 4′,6-diamidino-2-phenylindole (DAPI) as a nuclear dye. DIC: differential interference contrast. Images shown are representative of at least 30–50 images scanned for each stage from three sets of IFA labeling experiments. Shrivastava D, Jha A, Kabrambam R, Vishwakarma J, Mitra K, Ramachandran R, Habib S. Plasmodium falciparum ZIP1 Is a Zinc-Selective Transporter with Stage-Dependent Targeting to the Apicoplast and Plasma Membrane in Erythrocytic Parasites. ACS Infect Dis. 2024 10(1):155-169.