PfEIF3i is expressed in all intra-erythrocytic stages and localizes to the cytoplasm. Immunofluorescence assays of fixed RBCs infected with Plasmodium falciparum NF54 or PfEIF3i-HARibo parasites clones C7 and F12 at ring (R), trophozoite (T), and schizont (S) stages. EIF3i-HA was detected using anti-HA antibodies (green), and DNA was stained with DAPI (blue).

Dobrescu I, Hammam E, Dziekan JM, Claës A, Halby L, Preiser P, Bozdech Z, Arimondo PB, Scherf A, Nardella F. Plasmodium falciparum Eukaryotic Translation Initiation Factor 3 is Stabilized by Quinazoline-Quinoline Bisubstrate Inhibitors. ACS Infect Dis. 2023