Expression of EXP2 under the ptex150 promoter affects parasite growth but not protein export. (C) Representative IFA (n = 3) of infected erythrocytes with DAPI staining (blue) marking the parasite’s nucleus shows that endogenous EXP2 (HA) and cMyc-tagged EXP2 co-localize. (F) IFA of PfEXP2-HAglmS/pPTEX150 5′-EXP2-cMyc–infected erythrocytes using antibodies to the Maurer’s cleft protein PTP2 and quantification of PTP2 localization (n = 30 cells from a single experiment), defined as either fully exported, not exported, or showing an intermediate export phenotype. Scale bar, 5 μm.

Pitman EL, Counihan NA, Modak JK, Chowdury M, Gilson PR, Webb CT, de Koning-Ward TF. Dissecting EXP2 sequence requirements for protein export in malaria parasites. Front Cell Infect Microbiol. 2024 13:1332146. PMID: 38282616