Plasmodium falciparum (Pf) CZIF1 and PfCZIF2 are highly expressed in schizonts and localise in distinct puncta at different stages of development. Parasite cultures were saponin harvested at various hours post-invasion (h.p.i.) at rings (R, 0-6 h.p.i.), early trophozoites (ET, 12-18 h.p.i), late trophozoites (LT, 24-32 h.p.i.) and schizonts (S, 40-44 h.p.i. with E64 treatment) to determine the protein expression profile for each of (A) PfCZIF1 and (C) PfCZIF1 and (D) PfCZIF2 at the same time points and imaged by confocal microscopy. Blue, DAPI; green, anti-HA (AlexaFluor488); red, anti-EXP2 (AlexaFluor 555); magenta, anti-MSP1-19 (AlexaFluor 694). Images are representative of n = 3 biological replicates.

Balbin JM, Heinemann GK, Yeoh LM, Gilberger TW, Armstrong M, Duffy MF, Gilson PR, Wilson DW. Characterisation of PfCZIF1 and PfCZIF2 in Plasmodium falciparum asexual stages. Int J Parasitol. 2022 15:S0020-7519(22)00152-7. Epub ahead of print. PMID: 36400305