Newly invaded ring-stage parasites were probed with the mouse monoclonal anti-MSP119 (PFI1475w) antibody 1E1, and either rabbit anti-CTD (C-terminal domains; top panel) or anti-NTD (N-terminal domains; lower panel) antisera. Mouse antibodies were detected with an anti-mouse Alexa Fluor 594 conjugate (red) and rabbit antibodies with an anti-rabbit Alexa Fluor 488 conjugate (green). Parasite nuclei were stained with DAPI (blue). Scale bars are 2 μm. There is a clear reactivity of anti-CTD antibodies, but not anti-NTD antibodies, with ring-stage parasites. Anti-CTD and anti-MSP119 signals appear to co-localize (i), suggesting that ‘stub’ part of GAMA that is produced upon shedding of the p42-p37 complex is carried into the host cell and remains associated with the ring-stage parasite. Scale bar 2 μm.

Hinds L, Green JL, Knuepfer E, Grainger M, Holder AA. A novel putative GPI-anchored micronemal antigen of Plasmodium falciparum that binds to erythrocytes. Eukaryot Cell. 2009 8:1869-1879. Copyright 2010.